You generally don't need a "Path to Perl" on NT since the path to perl is included in the PATH variables at the system level. However, I do code the full path anyway as a reference. (E:\perl\bin\perl.exe)
no need for a path at all. i think you need to keep this line in just to tell the server it's a perl script, but wouldnt stake my life on it. i do a lot of perl coding on windows and always keep this line in like this and all works fine.