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c:\windows directory\Microsoft .NET\Framework\v1.1.4322\aspnet_regiis -i
This modifies the IIS metabase to know about .NET. If you install dotnetfx, that actually just reinstalls the .NET framework, which is unnecessary. The v1.1.4322 in the path above should be replaced by the highest version number you have installed of the framework.
aspnet_regiis can be run at any time to fix the IIS metabase to know about .NET. There is no harm in running it multiple times.