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However, prolonged standing has some issues too: sore feet, lower back and neck (muscles used to support standing). Here's a good article on working in a standing position [ccohs.ca]
My conclusion is that alternating working positions is the healthiest option we have (if we must keep working:). I recently bought a very cool height adjustable desk that, at the push of a button goes up and down allowing for working both sitting on a chair and standing. I sit for 2-3 hours with 5 minute breaks every half hour, than I stand for 2 hours, again with half hour breaks and so on. The 5 minute breaks are also good for your eyes. On these breaks I always try to focus my eyes on a distant object (it's what opticians recommend).
One other thing I intend to purchase is one of those $500 ergonomic chairs.
I don't have back pain or feet that hurt but I attribute that to getting away from the work place on the bike, doing grocery etc., meeting folks, and using the eyeballs to look at distant objects, ie.: cars that might run me down! :)
And there's nothing wrong with having the perfect chair! Best investment one can make in this kind of business.