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keep on tryin!
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[edited by: limbo at 3:46 pm (utc) on Dec. 10, 2007]
Or, just re-open the .gif and look at it, you will see the non-transparent line and be able to determine what's doing it in the original file.
A good example of this is the smilies common to many forums, they have sets for light or dark backgrounds. The key is getting the correct color soft edge.