Vhost Directory directives in apache2.conf

proper placement of Directory directive in Vhost environment

[krausdogs]

In an Ubuntu Apache2 virtual hosting environment I’m wanting to know if it is important that the Directory directive be placed in each vhost file sitting in the sites-available folder, Or can it simply be placed in the apache2.conf file.
The directive I'm referring to looks like:

<Directory />
Options FollowSymLinks
AllowOverride None
</Directory>

and is often place in the vhost file as such:

<VirtualHost *:80>
ServerAdmin webmaster@localhost
ServerName site1.wise.bob.com
DocumentRoot /var/www/mydrupalsites
<Directory />
Options FollowSymLinks
AllowOverride None
</Directory>
<Directory /var/www/mydrupalsites>
...

If ok to have in apache.conf, at what location should it be placed? Above or below the following?

<VirtualHost *:80>
ServerName wise.bob.com
DocumentRoot /var/www/
DirectoryIndex index.html
</VirtualHost>

Here?

# Include the virtual host configurations:
Include /etc/apache2/sites-enabled/

Here? Does it matter?
Thanks