I think you're talking at cross purposes :)
The root may or may not be a physical file that already exists. That part doesn't matter. What matters is what the user gets when they ask for the root.
Right now you've got what linguists call double markedness: :32400 AND /web
What happens if someone requests (explicitly, by type-in)
or, for that matter,
Do you want your alternative access routes to work instead of or in addition to the existing form? (This is probably an SEO question rather than an Apache question. The answer depends on whether this is a publicly accessible, indexed area, or something available only to people who know about it.)
My first reaction to your initial post was "Can't you just redirect?" That would be an alternative to futzing around with aliasing and listening.
I assume this isn't a Plex question, since there are a gazillion pages that talk about that.